High Performance Liquid Chromatography

Photography by Aleksandra Nedovic, Charles E. Schmidt College of Science

The photo shows the inside of the very sensitive instrument used in our laboratory daily when determining the purity of our compounds. High-Performance Liquid Chromatography (HPLC) allows quantitative and qualitative measures of compounds and how much of the compound is present. Auto-sampler vials shown in the picture carry samples of interest. Samples tested are in liquid form and volumes are in μL. It can run with small sample volumes lower than 2 μL and as high as 200 μL. The picture also shows a multi-well plate that can carry 96 or 384 samples and test them individually. You can test organic and aqueous solvents that contain the sample for HPLC.
Mentor: Predrag Cudic, Ph.D., Charles E. Schmidt College of Science