Pompano Culture in Recirculating Aquaculture Systems

Slide 1: Title slide for Pompano Culture in Recirculating Aquaculture Systems presentation by Paul S. Wills

Slide-1

Pompano Culture in Recirculating Aquaculture Systems

Paul S. Wills

Funding Thanks to Florida Department of Agriculture, Division of Aquaculture

Exterior view of USDA and Harbor Branch FAU inland aquaculture research facility featuring five large white Quonset-style buildings.

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Title: Inland Recirculating Aquaculture Systems

This is an aerial-level photograph of a research complex located in a flat, coastal landscape. Five long, white, arched Quonset-style buildings are lined up in a row. Overlaid blue text identifies the functions of the buildings: Production, Reproduction and Larviculture, Nutrition, and Engineering. At the bottom, logos for the USDA Agricultural Research Service (ARS) and Harbor Branch Oceanographic Institute at Florida Atlantic University are displayed.

Slide 3: List of benefits of Recirculating Aquaculture Systems

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Recirculating Aquaculture Systems (RAS)

  • Year round growing season
  • Reduced water consumption
  • Reduced effluent discharge
  • Intensive production
  • Enhanced biosecurity
  • Increased food safety
  • Containment of escapees
  • Environmental Sustainability
Slide 4: Diagram showing basic RAS design with filtration components and water flow

Slide-4

Basic RAS Design

The diagram illustrates the basic recirculating aquaculture system design showing the flow of water and waste treatment processes. Key components include:

Solids Filtration: Removes solid and dissolved wastes from the system.

Biofiltration: Converts ammonia (highly toxic) to nitrate (very low toxicity).

System Inputs: Makeup water & evaporation, oxygen, and feed enter the system.

System Outputs: Waste water and concentrated waste sludge exit the system.

The water continuously recirculates through the filtration systems to maintain water quality for the fish.

Slide 5: Information about advanced RAS design and operation workshop

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Advanced RAS design and Operation

HBOI-FAU ACTED Workshop:
"Recirculating Aquaculture Systems: Design, Engineering and Operation"

Instructors: Michael Timmons and Jim Ebeling
Dates: October 17-19, 2013
Cost: $450

Information: See Jill Sunderland
or, www.aquaculture-online.org
or, www.fau.edu/hboi
or, call 772-242-2506

Slide 6: Description of the basis for commercial system design and testing results

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Basis of the Commercial System Design

  • Scaled up version of tested design developed jointly for USDA-ARS project and FL-FWCC marine hatchery program FMFEI
  • System Tested with:
    • Red Drum up to ~90 Kg/m3
    • Florida Pompano ~40 Kg/m3
Slide 7: Diagram and photo of low head growout system with numbered components

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LOW HEAD GROWOUT SYSTEM

System components labeled 1-7:

  1. Long flow pathway moving bed reactor with cross flow oxygenator, float valves, and propeller pump
  2. Incoming salt and freshwater lines with float valves and water meters
  3. UV sterilizer
  4. Torrus filters with 13ft3 of MB3 floating plastic media
  5. Ten-foot diameter tanks w/ center sump and sidebox drain
  6. Diverter box
  7. 60 micron drum filter

The image shows both a schematic diagram and a photograph of the actual system installation.

Interior view of an aquaculture facility showing a large blue rectangular tank and complex PVC piping systems.

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TIndustrial aquaculture equipment. In the foreground is a large, rectangular blue tank with white PVC framing on top, divided into several sections with visible dark sediment or bio-matter. Extensive white PVC plumbing runs along the walls and ceiling. In the background, there is a blue mechanical filtration unit and several large circular tanks.

Slide 9: Graph showing biofilter volumetric nitrification rates

Slide-9

Long Flow Pathway Moving Bed Biofilter

BIOFILTER VOLUMETRIC NITRIFICATION RATES

The graph displays the relationship between influent TAN (Total Ammonia Nitrogen) concentration and nitrification rates in the moving bed biofilter.

X-axis: Influent TAN (mg/L), ranging from 0.00 to 1.60

Y-axis: g TAN/m3 media-day, ranging from 0.0 to 175.0

Data trend: The graph shows an increasing nitrification rate as influent TAN concentration increases, demonstrating the biofilter's capacity to process ammonia. The rate increases from near zero at low TAN concentrations to approximately 125-150 g TAN/m3 media-day at higher influent TAN concentrations around 1.4-1.6 mg/L.

Slide 10: Schematic diagram of commercial system design showing all components

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Commercial System Design

Not to scale

* Static Bed filters each contain 8 ft3 of MB3 biomedia

The diagram shows a comprehensive commercial RAS layout including culture tanks, moving bed biofilters, static bed filters, drum screen filter, UV sterilizers, foam fractionator, oxygen injection systems, and water flow pathways connecting all components.

Slide 11: Panoramic photograph of the filtration system showing various components labeled

Slide-11

Panorama of Filtration System

The photograph shows the complete filtration system with labeled components including:

  • Biofilter
  • Drum Screen Filter
  • Foam Fractionator
  • Main Pumps
  • Return Water Pumping
  • Static Bed Filters
  • Ultraviolet filters

The image provides a real-world view of how these components are arranged in the operational system.

Slide 12: Photograph of the drum screen filter component

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Drum Screen Filter

The image shows a close-up view of the drum screen filter, which is a cylindrical rotating filter used to remove solid waste particles from the water.

Slide 13: Photograph showing the microscreen cleaning process in action

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Microscreen Cleaning Process

The image captures the microscreen filter during its cleaning cycle, showing water spray removing accumulated solids from the screen surface.

Slide 14: Description of waste water recapture system using static bed filters

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Waste Water Recapture

  • We used a Static Bed Filter to separate solids from Drum Screen Filter Waste stream
    • Concentrates solids prior to discharge
    • Recaptures significant component of water used for wash down of drum screen
    • Each contains 8 Ft3 MB3 Media
Slide 15: Bar charts comparing solids removal efficiency across different filter types

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Solids removal efficiency

RE = (TSSIN-TSSOUT) / TSSIN

The slide contains two bar charts showing removal efficiency percentages:

Chart 1: Overall Solids Removal Efficiency

Filter Type Removal Efficiency (%)
Swirl separator 14.9 ± 17.4
Static bed 43.2 ± 12.0
Moving bed 10.3 ± 8.5

Chart 2: Solids removal efficiency (< 50 microns)

Filter Type Removal Efficiency (%)
Swirl separator -6.5 ± 2.1
Static bed 22.2 ± 1.7
Moving bed 6.8 ± 2.1

The static bed filter shows the highest removal efficiency for both overall solids and fine particles less than 50 microns.

Slide 16: Photograph of moving bed biofilter with aeration

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Moving Bed Biofilter (250 ft3 MB3 Biomedia)

Aeration Keeps Bed Fluidized

The image shows the moving bed biofilter in operation with visible aeration bubbles keeping the plastic biomedia in constant motion for optimal biological filtration.

Slide 17: Photograph of UV sterilization units with dosage information

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UV Sterilization

Minimum Dose Desired
30,000 uW sec/cm2

The two units delivering
~150,000 uW sec/cm2

The photograph shows two large UV sterilization units that provide significantly higher dosage than the minimum required for effective pathogen control.

Slide 18: Photograph showing tank covers on the culture system

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Tank Cover

The image displays the covered culture tanks, which help maintain stable environmental conditions and reduce evaporation.

Collage of five images showing aeration and oxygenation equipment, including monitoring displays and air diffusers.

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Aeration and Oxygenation.

A collage shows various technical components: 1) A wall-mounted control panel with numerous gray tubes and wires. 2) A close-up of a YSI 5200 monitoring screen showing Dissolved Oxygen at 89.3% and Temperature at 28.2°C. 3) Several long, rectangular white ceramic air diffusers sitting on a wooden pallet. 4) A large industrial regenerative blower motor. 5) A flexible white "soaker" style aeration hose on a wooden deck.

Slide 20: Diagram showing aeration and oxygenation zones in a 25-foot diameter tank

Slide 20

Aeration and Oxygenation

25 ft Diameter

The diagram illustrates a circular tank divided into three zones:

  • Oxygenation Zone 1: Primary oxygen injection area
  • Aeration Zone: Central area with air diffusion
  • Oxygenation Zone 2: Secondary oxygen injection area

O2 Sensors are positioned to monitor dissolved oxygen levels throughout the tank. The layout ensures optimal oxygen distribution for fish health and growth.

Slide 21: Introduction to backup systems and Murphy's Law

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Backup Systems

  • Systems designed to combat "Murphys Law"
    • "If anything can go wrong it will"
  • Therefore managers must:
    • Anticipate
    • Plan
    • Train
    • Respond
  • (an automatic system that "cries wolf" can derail this necessary task)
Slide 22: List of backup systems including generators and automatic oxygen

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Backup Systems

  • Generators
  • Automatic oxygen system
Slide 23: Photograph of generator with requirements information

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Generators

  • Must have disconnect switch and meet other local requirements

The image shows an installed backup generator system for the facility.

Close-up of industrial electrical control panels and internal circuit boards for the aquaculture system.

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Two main control boxes are mounted on a blue wall. The left box is a large, dark gray double-door metal enclosure with a small digital status display. To the right is a smaller white box with a transparent window showing four digital controllers and organized red wiring. An inset photo shows the interior of a panel filled with complex electrical components, including rows of blue relays, circuit breakers, and a programmable logic controller (PLC).

Slide 25: Diagram and explanation of automatic oxygen system with failsafe design

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Automatic Oxygen System

  • Opens oxygen flow to tanks regardless of monitoring systems or other backup systems if the power turns off
  • Must use Normally Open Solenoid Valves for failsafe system
    • Normally open closed when power applied

The slide includes a diagram showing the oxygen system configuration with normally open solenoid valves that ensure oxygen continues to flow to tanks during power failures.

Slide 26: Title slide for broodstock conditioning and spawning system

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Broodstock Conditioning and Spawning System

USDA-ARS/HBOI-FAU Design

Slide 27: Photograph and specifications for Florida Pompano broodstock

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Broodstock Profile

Florida Pompano

Weight: 0.7 – 2.3 kg
Mature age: 1- 3 yrs
Temp: 18 – 30 C
Salinity: 5 – 36 ppt

The slide includes a photograph of Florida Pompano fish.

Slide 28: Table of water quality management parameters and target levels

Slide-28

Water Quality Management Parameters

Parameter Target Level
Temperature 16 – 30 C
pH 7.6 – 8.2
Dissolved oxygen ≥ 5 mg/L
Alkalinity ≥ 200 mg/L
Ammonia ≤ 1 mg/L
Nitrite ≤ 2 mg/L
Total dissolved solids ≤ 5 mg/L
Slide 29: Diagram and photographs of broodstock conditioning system components

Slide-29

Broodstock Conditioning System

The slide shows a flow diagram of the broodstock conditioning system with the following components and volumes:

  • Sump (2.3 m3)
  • Culture Unit (5.7 m3)
  • Egg Collectors (0.3 m3)
  • Beadfilter (0.1 m3)
  • Pump to chiller
  • Cartridge filters
  • Return from chiller
  • UV sterilization
  • Waste outlets at multiple points

The diagram illustrates the complete recirculation pathway for maintaining optimal conditions for broodstock spawning. The slide also includes photographs of the actual system components.

Interior aisle of a facility lined with two rows of large, black circular fish tanks covered with white lids.

Slide 30

A central metal grate walkway runs between two rows of large, black cylindrical aquaculture tanks. Each tank is topped with a white, slightly domed cover. An orange industrial floor fan sits in the aisle. The room has arched, insulated ceilings and a silver metal roll-up door at the far end of the hallway.

Collage of aquaculture maintenance tasks including tank lighting, HVAC units, and water filtration using mesh bags.

Slide 31

A collage of five images showing system maintenance: 1) A circular tank with a blue light glowing from under a partially lifted lid. 2) Three Trane HVAC condenser units installed outside the white building. 3) Water flowing from white PVC pipes into blue rectangular filter basins. 4) A close-up of a worker holding a mesh filter bag used to capture waste or larvae from a water outlet. 5) Water rushing into a smaller filter box with a mesh screen.

Slide 32: Photograph of larviculture system

Slide 32

The photograph displays the Larviculture system equipment with inset closeup of top of a large tank

Slide 33: Photograph of nursery system tanks

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Nursery System

The photograph displays the nursery system tanks where juvenile pompano are grown before transfer to the main growout system.

Slide 34: Discussion of RAS technology limitations related to waste management

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Limitations of RAS Technology

  • Waste products from the fish must be treated before discharge
    • This is much easier than in other aquaculture systems such as open ocean net-pens
  • No one has ever achieved 100% recirculation due to water needed for removing waste products and lost to evaporation

WASTES !!!!!

Slide 35: Discussion of waste characteristics and nutrient content

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WASTES!!! WHAT?! WASTE !!!!!

  • What are they?
    • Uneaten feed and other Solids
    • Liquid Excretions
  • One Characteristic of these "Wastes" is that they contain quite a bit of nutrient
    • (esp., Nitrogen, Phosphorus)
  • So, why not USE nutrients instead of discharging them?
Slide 36: Introduction to Integrated Multi-Trophic Aquaculture as a solution

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The Answer:

Integrated Multi-Trophic Aquaculture (IMTA)

Turn Wastes into Resources

The Next Generation in Recirculating System Design

IMTA = Integrated Multi-trophic Aquaculture Systems

Slide 37: Diagram showing prototype land-based IMTA system with different culture components

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Prototype Land Based HBOI-IMTA

Funded Through Aquaculture SLP

The diagram illustrates an integrated multi-trophic aquaculture system with the following components:

  • Primary fish culture receiving prepared feed
  • Processing solids from the fed culture
  • Organisms that consume and utilize settleable solid wastes
  • Organisms that filter and utilize suspended particles
  • Organisms that absorb dissolved nutrients from the water

End of presentation.
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Florida Atlantic's Harbor Branch Oceanographic Institute engages with the community through the Ocean Discovery Visitors Center and the Ocean Science Lecture Series. Harbor Branch’s research and outreach programs translate marine science in order to provide solutions that improve economies and quality of life for coastal communities.
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