Instruments & Equipment

Fluorescent Microscopy

Keyence BZ-X710 All-in-One Fluorescence Microscope
Keyence BZ-X710 All-in-One Fluorescence Microscope

Location: FAU Jupiter

Overview:
Compact easy-to-use fluorescent microscope with high resolution monochrome CCD camera. With its fully motorized XYZ stage large samples can be captured and stitched fully automatically.

Confocal Microscopy

Nikon A1R Confocal System on an Eclipse Ni Upright Microscope with Spectral Detector
Nikon A1R Confocal System on an Eclipse Ni Upright Microscope with Spectral Detector

Location: FAU Jupiter

Overview:
The Nikon A1R confocal microscope is an easy to operate imaging system, used for a wide range of imaging applications. In addition to the four standard detectors, the system is equipped with a spectral detector enabling acquisition of up to 32 channels simultaneously.

Nikon A1R Confocal System on an Eclipse Ni Upright Microscope
Nikon A1R Confocal System on an Eclipse Ni Upright Microscope

Location: FAU Jupiter

Overview:
The Nikon A1R confocal microscope system allows for the acquisition of four fluorescent channels simultaneously. For fast image acquisition the resonant scanner can achieve scanning speeds of up to 420 fps. Mounted on an upright microscope stand, this is the preferred system for fixed sample slides.

Nikon AXR Confocal System on an Eclipse Ti2-E inverted Microscope
Nikon AXR Confocal System on an Eclipse Ti2-E inverted Microscope

Location: FAU Jupiter

Overview:
The Nikon AXR confocal microscope combines fast scanning with a large field of view. Mounted on an inverted microscope stand and equipped with four laser lines and four detectors, this system offers high versatility for a wide range of imaging applications.

Super-Resolution Microscopy

Nikon A1X Confocal Microscope with NSPARC on a Ti-E Inverted Microscope
Nikon A1X Confocal Microscope with NSPARC on a Ti-E Inverted Microscope

Location: FAU Jupiter

Overview:
The Nikon AXR with NSPARC imaging system provides high-resolution imaging with exceptionally low noise. Its specialized detector enables an improved signal-to-noise ratio, allowing high-quality image acquisition at much lower excitation power. This helps preserve fluorescence and enables gentler imaging of live samples.

Nikon N-SIM E Super-Resolution System with A1+ Confocal Microscope on the Eclipse Ti2 Inverted Microscope
Nikon N-SIM E Super-Resolution System with A1+ Confocal Microscope on the Eclipse Ti2 Inverted Microscope

Location: FAU Boca Raton

Overview:
This system combines confocal and super-resolution capabilities in one setup. Visualization of small intracellular structures and their function can be achieved using structured illumination (SIM) technology. The N-SIM E realizes double the spatial resolution of conventional optical microscopes (to approximately 115 nm)

Multi-Photon Microscopy

Nikon A1R Multiphoton with FN1 Upright Microscope
Nikon A1R Multiphoton with FN1 Upright Microscope

Location: FAU Jupiter

Overview:
Nikon's A1R multiphoton microscope enables high speed, deep tissue imaging in live organisms with great sensitivity and clarity. The system features an ultra-high speed resonant scanner capable of up to 420 fps.

Light-Sheet Microscopy

Miltenyi UltraMicroscope Blaze
Miltenyi UltraMicroscope Blaze

Location: FAU Jupiter

Overview:
For fast acquisition of large samples such as whole mouse brains, organs, or other cleared tissues, the UltraMicroscope Blaze is equipped with LightSpeed Mode. This light-sheet microscope is highly user-friendly and enables imaging of up to four different fluorescent channels.

Digital Spatial Profiler

nanoString GeoMX Digital Spatial Profiler
nanoString GeoMX Digital Spatial Profiler

Location: FAU Jupiter

Overview:
The GeoMx DSP is a flexible and scalable platform for spatial biology. GeoMX is the only spatial biology platform that non-destructively profiles expression of RNA and protein from distinct tissue compartments and cell populations with an automated and scalable workflow that integrates with standard histology staining. Spatially profile the whole transcriptome and 570+ protein targets separately or simultaneously from your choice of sample inputs: whole tissue sections, tissue microarrays (TMAs), or organoids.

Image Analysis Workstations

The Advanced Cell Imaging Core offers a variety of image analysis workstations for 2D and 3D image analysis. The following software tools are available free of charge for users of the Core:

Nikon NIS-Elements Advanced Research with 2D/3D Deconvolution module

Oxford Instruments Imaris 3D/4D Image Analysis Software

MBF Bioscience Neurolucida Neuron tracing and analysis software

MBF Bioscience NeuroInfo (whole brain investigation of circuits, neuronal populations, and biochemical marker expression)

Miltenyi Biotec MACS® iQ View – 3D Large Volume