Picture depicts the brain of the fruit fly. Expression of cytosolic green fluorescent protein (GFP) and mCherry-tagged Distracted protein happening simultaneously under the control of the Distracted gene (Dsd). The regulatory sequences show its gene [removed]in green) and protein localization (magenta), with the pink coloring representing flowers, and gene expression the leaves of a bouquet. The labeling reveals that the protein is most abundant in somas and the lack of co-labeling (showing together of) GFP in some somas implies that the protein negatively regulates its own gene expression. This image was taken with a 20x objective lens using a Nikon AR1 confocal microscope.