Education
Doctor of Philosophy in Immunology, January 2004
University of Texas Graduate School of Biomedical Sciences and MD Anderson Cancer Center, Houston, Texas
Dissertation: Effects of the microenvironment on Fas and Fas ligand interactions and their implications on UV-induced immune suppression
Bachelor of Science, May 1996 Summa Cum Laude
Salem-Teikyo University, Salem, West Virginia.
Major: Molecular Biology/Biotechnology
Minors: Chemistry and European Studies
Related Experience
Assistant Research Professor
December 2007-present
Harbor Branch Oceanographic Institute, Fort Pierce, Florida
Assistant Research Scientist
February 2007-December 2007
Harbor Branch Oceanographic Institution, Fort Pierce, Florida
Participate as a senior member of a multidisciplinary team aimed at the discovery of marine-derived therapeutics. Provide insight and expertise in cell biology to the team. Develop cell-based assays to determine the potential therapeutic effects of the library of marine compounds to fight cancer and other diseases. Determine the mechanism of action of compounds that exhibit potential as therapeutics. Provide research advice and supervision for technicians and students. Write competitive grant applications, publications, and patent applications. Present research at scientific meetings. Oversee equipment and laboratory.
Postdoctoral Investigator
May 2005-February 2007
Harbor Branch Oceanographic Institution, Fort Pierce, Florida
Develop cell-based assays to determine the potential therapeutic effects of our library of marine compounds to fight cancer and other diseases. Develop an assay to determine if any of our compounds could inhibit pMEK, a molecule that confers metastatic and anti-apoptotic abilities to cancer cells. Evaluated the compounds found to be inhibitors to determine their therapeutic potential. Establish new techniques for the high-throughput screening of induction of apoptosis by our compounds, and assays to determine the ability of our compounds to restore p53 activity and to increase the sensitivity to apoptosis of pancreatic cancer cells. Determine the mechanism of action of compounds that are known to kill pancreatic cancer cells. Provide research advice and supervision for technicians and students. Write competitive grant applications. Present research at scientific meetings.
Postdoctoral Fellow
April 2004-May 2005
Henry Ford Health System, Detroit, Michigan
Conducted research in immunology and cancer biology studying the regulation of UV-induced apoptosis by the microphthalmia associated transcription factor in melanocytes. Determined that the levels of expression of MITF inversely regulate the ability of melanocytes to undergo apoptosis, which may be of importance in the development of melanoma. Isolated and maintained primary cultures of melanocytes from wild-type and transgenic animals. Performed western blotting to ascertain expression and function of proteins from the apoptotic machinery. Provided research advice and supervision for technicians and students. Wrote competitive grant applications. Presented research at scientific meetings.
Graduate Research Assistant
September 1997-2004
University of Texas MD Anderson Cancer Center-Houston, Texas
University of California, Riverside, California
Conducted research in immunology and cancer biology studying the effects of Interleukin-10 on Fas and Fas ligand interactions on T cells to understand their role in the phenomenon of ultraviolet light-induced immune suppression. Assayed immunological functions of short term-cultured splenocytes from wild-type, knockout and transgenic animals. Performed magnetic separation of T cell subsets. Analyzed expression of cell surface markers through flow cytometry. Ascertained expression of Fas ligand through RT-PCR and intracellular staining, of IL-10 through ELISA assays and intracellular staining, and of other cytokines through protein arrays. Performed immunohistochemistry on epidermal sheets to analyze morphology of Langerhans cells and Dendritic Epidermal T cells after exposure to ultraviolet light, visualized by bright field or confocal fluorescence microscopy. Performed western blotting and immunoprecipitations to ascertain expression and function of proteins from the apoptotic machinery. Performed colorimetric analysis to determine cleavage of caspases and viability of cells. Assisted in relocating the laboratory from Houston to Riverside. Provided research advice and supervision for junior graduate students and undergraduates.Presented research at scientific meetings. Contributed as lecturer for a graduate seminar series.
Graduate Research Assistant
September 1996-August 1997
Los Alamos National Laboratory, Los Alamos, New Mexico
Theoretical Biology and Biophysics Division; HIV Database
Determined the correlation of areas of homology among genetic sequences of different subtypes of HIV-1 and conserved epitopes of known immunogenicity. Analyzed genetic sequences to determine homology among sequences. Performed phylogenetic analysis of sequences. Read and summarized research articles on HIV. Updated sequence data bank. Assisted in creating alignments and the consensus sequence of the sequences in the database. Helped maintain the UNIX-based web page of the database. Co-authored a book chapter. Presented research at intradepartmental seminar.
Independent Researcher
May 1995- May 1996
Salem-Teikyo University, Salem, West Virginia
Isolated genomic DNA from plant leaves. Performed restriction enzyme digestion of DNA and Southern Blot analysis. Designed PCR primers. Transfected cells with plasmids. Performed sequence comparisons to determine the degree of homology of polyphenol oxidase from different plants. Presented research at a state scientific meeting, as well as intradepartmental seminars.
Student Fellow
Howard Hughes Medical Institute Summer Fellowship 1993
West Virginia University, Morgantown, West Virginia
Performed cell culture of HeLA cells labeled with tritiated thymidine. Exposed cells to the benzene metabolites phenol and 1,2,4 benzenetriol.Nicked DNA with Endonuclease III and performed sucrose gradient separation of DNA. Used a scintillation counter to determine formation of pyrimidine dimers. Presented research at the fellowship seminar.
Reviewer
Anti-Cancer drugs 2012
NIH Early Career Reviewer Cancer Health Disparities Study Section 2011
Marine drugs 2011
BMC Cancer 2010
Biotechnology and Applied Biochemistry 2009
Letters in Drug Discovery 2009
Anti-Cancer Drugs 2009
Ad hoc reviewer, American Cancer Society 2002
Molecular Carcinogenesis 2000
Read, criticized content and presentation, and advised on scientific importance of grants submitted for funding or articles to appear in scientific journals.
Patents
Wright A., Sennett S., Pomponi S., McCarthy P., Guzmán E. Biologically Active Aphrocallistin Compounds and Methods of Use. U.S. Patent No. 8,058,430 Issued: November 15, 2011
Guzmán, E.A.; Johnson, J.D. and Wright, A.E. Use of Manzamine Compounds in Anti-cancer Therapeutic Regimens. U.S. Patent No. 8,232,266. Issued: July 31, 2012
Publications
Peer Reviewed
:
Winder PL, Baker HL, Linley P, Guzmán EA, Pomponi SA, Cristina Diaz M, Reed JK, Wright AE. Neopetrosiquinones A and B, sesquiterpene benzoquinones isolated from the deep-water sponge Neopetrosia cf. proxima. Bioorg Med Chem. 19(22):6599-6603, 2011.
Paterson I, Dalby SM, Roberts JC, Naylor GJ, Guzmán EA, Isbrucker R, Pitts TP, Linley P, Divlianska D, Reed JK, and Wright AE. Leiodermatolide, a potent antimitotic macrolide from the marine sponge Leiodermatium sp. Angew. Chem. Int. Ed. 50(14):3219-3223, 2011.
Guzmán EA, Johnson JD, Linley PA, Gunasekera SE, Wright AE. A novel activity from an old compound: Manzamine A reduces the metastatic potential of AsPC-1 pancreatic cancer cells and sensitizes them to TRAIL-induced apoptosis. Invest New Drugs. 29(5):777-785, 2011
Paterson I, Naylor GJ, Gardner NM, Guzmán E, Wright AE. Total Synthesis and Biological Evaluation of a Series of Macrocyclic Hybrids and Analogues of the Antimitotic Natural Products Dictyostatin, Discodermolide, and Taxol. Chem Asian J. 6 (2):459-473, 2011.
Paterson I, Naylor GJ, Fujita T, Guzmán E, Wright AE. Total synthesis of a library of designed hybrids of the microtubule-stabilising anticancer agents taxol, discodermolide and dictyostatin. Chem Commun (Camb) 46(2):261-263, 2010.
Isbrucker RA, Guzmán EA, Pitts TP, Wright AE. Early Effects of Lasonolide A on Pancreatic Cancer Cells. J Pharmacol Exp Ther 331(2):733-739, 2009.
Wright AE, Roth GP, Hoffman JK, Divlianska DB, Pechter D, Sennett SH, Guzmán EA, Linley P, McCarthy PJ, Pitts TP, Pomponi SA, Reed JK. Isolation, Synthesis, and Biological Activity of Aphrocallistin, an Adenine-Substituted Bromotyramine Metabolite from the Hexactinellida Sponge Aphrocallistes beatrix. J Nat Prod. 72(6):1178-1183, 2009.
Hornyak TJ, Jiang S, Guzmán EA, Scissors BN, Tuchinda C, He H, Neville JD, Strickland FM. Mitf Dosage as a Primary Determinant of Melanocyte Survival after UV Irradiation. Pigment Cell Melanoma Res. 22(3):307-318, 2009.
Paterson I, Gardner NM, Guzmán E, Wright AE. Total synthesis and biological evaluation of novel C2–C6 region analogues of dictyostatin. Bioorg Med Chem 17(6):2282-2289, 2009.
Buhler LA, Samara R, Guzmán E, Wilson CL, Krizanac-Bengez L, Janigro D, and Ethell DW. Matrix metalloproteinase-7 facilitates immune access to the CNS in experimental autoimmune encephalomyelitis. BMC Neurosci 10:17, 2009.
Guzmán EA, Johnson JD, Carrier MK, Meyer CI, Pitts TP, Gunasekera SP, Wright AE. Selective cytotoxic activity of the marine derived Batzelline compounds against pancreatic cancer cell lines. Anticancer Drugs 20(2):149-155, 2009.
Paterson I, Gardner NM, Guzmán E, Wright AE. Total synthesis and biological evaluation of potent analogues of dictyostatin: Modification of the C2–C6 dienoate region. Bioorg Med Chem Lett 18(23):6268-6272, 2008
Guzmán EA, Langowski JL, De Guzman A, Muller HK, Walker AM, Owen LB. S179D prolactin diminishes the effects of UV light on epidermal gamma delta T cells. Mol Cell Endocrinol
280(1-2):6-12, 2008.
Ma LJ, Guzmán EA, DeGuzman A, Muller HK, Walker AM, Owen LB. Local Cytokine Levels Associated with Delayed Type Hypersensitivity Responses: Modulation by Gender, Ovariectomy and Estrogen Replacement. J Endocrinol 193(2):291-297, 2007.
Wright AE, Botelho JC, Guzmán E, Harmody D, Linley P, McCarthy PJ, Pitts TP,Pomponi SA, Reed JK. Neopeltolide, a Macrolide from a Lithistid Sponge of the Family Neopeltidae. J Nat Prod 70(3):412-416, 2007.
Tuchinda C, Lim HW, Strickland FM, Guzmán EA, Wong HK. Comparison of broadband UVB, narrowband UVB, broadband UVA and UVA1 on activation of apoptotic pathways in human peripheral blood mononuclear cells. Photodermatol, Photoimmunol & Photomed 23(1):2-9, 2007.
Ma LJ, Guzmán EA, DeGuzman A, Walter B, Muller HK, Walker AM, Owen LB. Unexpected effects of UVB in IL-10 transgenic mice: normalization of contact hypersensitivity response. Arch Dermatol Res 297(9):417-420, 2006.
Guzmán EA, Chen YH, Langowski JL, De Guzman A, Lo HL, Walter B, Muller HK, Walker AM, Owen LB. Abrogation of delayed type hypersensitivity response to Candida albicans produced by a molecular mimic of phosphorylated prolactin. J Neuroimmunol 170(1-2):31-40, 2005.
Guzmán Durán A, Guzmán E.A., Pannell K.H., and Lloyd W.D.. Synthesis of (E,Z)-5-Bromo-1,1,dimethosy-5-trimethylsilyl-4-pentene, an Upper Chain Allenic Prostaglandin Building Block. Synthetic Communications 33 (19), 3271-3283, 2003.
Reviews
:
E. Guzmán, J.L. Langowski, and L.B. Owen-Schaub. Mad Dogs, Englishmen and Apoptosis: The Role of Cell Death In UV-Induced Skin Cancer (Review). Apoptosis 8 (4) 315-325, 2003.
Book Chapters
:
C. Kuiken, B. Foley, E. Guzmán, B. Korber. Determinants of HIV-1 Protein Evolution. In The Evolution of HIV.pp. 432-468. Keith Crandall, Editor. John Hopkins University Press. Baltimore, MD, USA 1999.
J.C. Blouin, E.A. Guzmán, and B.T. Foley. Global Variation in the HIV-1 V3 Region. In G. Myers et al, Eds. Human Retroviruses and AIDS 1996, pp III 77-201, Los Alamos, NM. Los Alamos National Laboratory: Theoretical Biology and Biophysics, 1996.
Scientific Presentations
Lecture: E. Guzmán. Inflammation, Cancer and Marine Natural Products. Gordon Research Conference for Marine Natural Products. Ventura, CA 2012.
Poster: E. Guzmán, Maers K., Harmody D., Pitts T., Wright A. “Initial Studies on the Mechanism of NFκ B Inhibition by Microsclerodermin A.” American Association for Cancer Research (AACR) Tumor Microenvironment Complexity Conference November 3rd-6th, Orlando, Florida, 2011.
Poster: E. Guzmán, M. Maher, A. Temkin, T. Pitts, P. Linley and A. Wright. “Inhibition of Inflammation by Marine Natural Products.” Gordon Research Conference for Marine Natural Products, Ventura, CA 2010.
Poster: E. Guzmán, J. Johnson, P. Linley, S. Gunasekera and A. Wright. Manzamine A Reduces the Metastatic Potential of AsPC-1 cells and Sensitizes them to TRAIL Induced Apoptosis. 100th Annual Meeting of the American Association for Cancer Research, Los Angeles, CA 2007.
Poster: E. Guzmán, J. Johnson, C. Meyer, M. Carrier, T. Pitts, S. Gunasekera and A. Wright. Mechanisms of cytotoxicity Induced by Batzellines in pancreatic cancer cell lines. 9th Annual BioFlorida Statewide Conference, Gainesville, FL 2006.
Poster: E. Guzmán, C. Tuchinda, TJ Hornyak and FM Strickland. Regulation of UV-induced apoptosis in melanocytes by MITF. PanAmerican Society for Pigment Cell Research. Newport Beach, CA 2004.
Poster: E.A. Guzmán, L.L. Hill, and L.B. Owen-Schaub. IL-10 inhibits Fas/Fas Ligand mediated activation induced cell death in murine splenocytes. 27th La Jolla Immunology Conference, The Salk Institute, University of California San Diego; La Jolla, CA 2001.
Poster: E.A. Guzmán, L.L. Hill, and L.B. Owen-Schaub. IL-10 alters Fas/Fas Ligand induced cell death in activated lymphocytes. American Association of Immunologists and the Clinical Immunology Society Joint Annual Meeting. Seattle, WA 2000.
Poster: E.A. Guzmán and K. Cammarata. Polyphenol Oxidase Expression in Plant Genomic DNA. West Virginia Academy of Sciences. 1996. Winner of Poster Competition.
Lectures
Guzmán E. Sunken Treasure. Marine Drug Discovery for Pancreatic Cancer Treatment” Ocean Sciences Lecture Series. Harbor Branch Oceanographic Institute at FAU. March 28, 2012.
Guzmán E. FAU-VGTI Symposium at Florida Atlantic University“Cancer Cell Biology Group. Center for Marine Biomedical and Biotechnology Research.” October 14th, 2011.
Guzmán E. State of Florida Bankhead Coley Program Site Visit. Florida Atlantic University. “Discovery of novel anti-inflammatory compounds for use as chemo-preventatives of pancreatic cancer” May 2nd, 2011.
Guzmán E. Graduate Student Association 2nd Annual Research Day. Florida Atlantic University “Marine Drug Discovery: Bringing Cures to the Surface.” April 8th, 2011.
Guzmán E. Center for Molecular Biology and Biotechnology Seminar Series. Florida Atlantic University. “Finding novel inhibitors of inflammatory signaling pathways to prevent the initiation or progression of pancreatic cancer.” March 23rd, 2011.
Guzmán E. Nature Conservancy. Blowing Rocks Preserve, Jupiter, FL. “Marine Drug Discovery: Bringing Cures to the Surface.” March 26th, 2010.
Guzmán E. Department of Chemistry and Biochemistry Seminar. Florida Atlantic University. “Cures from the Depths of the Ocean: Tackling Inflammation to Fight Cancer.” February 10th, 2010.
Guzmán, E. Vero Beach Exchange Club, Vero Beach, FL. “Anti-cancer Drug Discovery from Marine Organisms—Bringing Cures to the Surface.” August 31st, 2009.
Guzmán, E. Ocean Sciences Lecture Series. Harbor Branch Oceanographic Institute. “Anti-cancer Drug Discovery from Marine Organisms—Bringing Cures to the Surface.” March 11th, 2009.
Guzmán, E. Ocean Health Colloquium Seminar. Harbor Branch Oceanographic Institute. “Anti-cancer Drug Discovery from Marine Organisms—The Cell Biology Perspective.” November 3rd, 2008.
Research Support
NIH R01CA093455-06(Wright): Discovery of Novel Compounds with Activity against Pancreatic Cancer. Total Award $990,160. Project period 7/01/10-6/30/13: Funded by: NIH RO1 Pilot Studies in Pancreatic Cancer. Role on Project: Co-investigator.
The major goal of this project is to discover bioactive marine natural products that lead to novel chemotherapeutics for the treatment of pancreatic cancer.
HBOI/FAU Postdoctoral Program (Guzmán). Identification of Novel Inhibitors of Inflammation to be used as Chemo-preventatives of Pancreatic Cancer. Total Award $66,488. Funded by HBOI Foundation. Project Period 09/27/10 to 09/26/12. Role on Project: Principal Investigator
This award provided funds to hire a postdoctoral investigator to assist me in my studies to identify novel inhibitors of inflammation that have the potential to prevent the initiation of pancreatic cancer.
NA09OAR4320073 (Pomponi) NOAA Cooperative Institute for Ocean Exploration, Research and Technology “Exploration of frontier habitats for new medicines.“ Total Award $204,402. Project Period: 07/01/09-06/30/11. Funded by NOAA. Role on Project: Co-investigator.
The major goal of this project is to discover bioactive marine natural products that lead to novel chemotherapeutics for the treatment of many diseases
NIH R03CA141199-01 (Guzmán). “Identification of bioactive marine natural products that inhibit mast cells implicated in pancreatic cancer etiology.” Total Award: $142,500. Project Period 07/01/09-06/30/11. Funded by NIH RO3 Pilot Studies in Pancreatic Cancer. Role on Project Principal Investigator
The overall objective of the proposed research project is to identify bioactive marine natural products that inhibit mast cell migration and degranulation as potential novel chemo-preventatives of pancreatic cancer.
09BN-08-23088 (Guzmán). “Discovery of Novel Anti-inflammatory Compounds for Use as Chemopreventatives of Pancreatic Cancer.” Total Award: $374,999. Project Period 7/1/09-6/30/12. Funded by: State of Florida Bankhead-Coley New Investigator Research Grant. Role on Project: Principal Investigator.
The major goals of this project are to screen the HBOI library of marine derived secondary metabolites in an effort to identify novel inhibitors of the signaling pathways NFκB, IL-8 and STAT3 which are of importance in inflammation and pancreatic cancer initiation/progression and to validate the activity of those samples through secondary assays.
NIH R56 CA093455-05 (Wright): Discovery of Novel Compounds with Activity against Pancreatic Cancer. Total Award: $488,611. Project period 8/22/08-6/30/10: Funded by: NIH RO1 Pilot Studies in Pancreatic Cancer. Role on Project: Co-investigator.
The major goal of this project is to discover bioactive marine natural products that lead to novel chemotherapeutics for the treatment of pancreatic cancer.
Governor’s Ocean Initiative. Drug Discovery from Florida’s Oceans. Total Award: $172,942. Project period 06/01/04-06/01/07. Funded by: State of Florida: Role on Project: Co-Investigator on Project 2.
Goals of the project: To discover compounds which induce apoptosis in tumor cells that exhibit resistance to programmed cell death (apoptosis).
Honors
2012 Florida Atlantic University Researcher of the Year, Assistant Professor Level
2010 Gordon Conference Carl Storm Underrepresented Minority Fellowship Recipient
2004 PanAmerican Society for Pigment Cell Research Travel Award Recipient
1996 Graduated Summa Cum Laude
1996 Outstanding Graduate Award
1996 West Virginia Academy of Sciences Poster Competition Winner
1995-1996 Salem-Teikyo University Student of the Year
1995 Okinaga Foundation Grant Recipient
1994-1996 Teikyo Foundation Scholarship
1993-1996 Salem-Teikyo University Student Administration Scholarship
1993 Howard Hughes Medical Institute Summer Fellowship
1992-1996 Salem-Teikyo University Scholarship
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